The major interest of our laboratory is to study organelle ion channels and transporters and understand their role in human and plant physiology and pathophysiology. We use a wide range of integrated approaches, including:
1) Organelle Electrophysiology: lysosome patch-clamp and parietal cell tubulovesicle patch-clamp.
2) Ca2+ imaging: using either genetically encoded Ca2+ sensor or Ca2+ sensing dyes such as FURA2-AM or FLUO4-3AM.
3) Ion channel pharmacology: small-molecule channel activators and inhibitors.
4) Cell biology: Immunohistochemistry, H & E staining, Fluorescence and Electron microscopy.
5) Mouse genetics: Ion channel transgenic (Loxp-cre-Loxp) and knockout mice.
6) Biochemistry: Western blot, pull-down assay, protein purification using HPLC, characterization by using several spectroscopic methods such as UV-Vis, Fluorescence quenching, Circular Dichroism spectroscopy.